Osmosis Lab Report

Fruit Osmosis Experiment Lab Report

Purpose

The purposes of this experiment are to find how juicy fruit/vegetable kiwi is between dragon fruits, rambutans, apples, and potatoes and to observe and study osmosis of cells because our cells are mostly made up of water and also depend on water to clean us, lubricate us, keep our shape, supply us oxygen, move nutrients, and act as enzymes to reactions.

Hypothesis

I do not think kiwis are either the juiciest fruit/vegetable or the driest fruit/vegetable because there are many other fruits and vegetables that seem juicier and drier. I also think the cells will explode once too much water gets inside.

Equipment

6 beakers, 1 electronic scale, water, glucose powdered, 18 pieces of kiwi, spoon, knife, timer

Procedures

  1. Fill the 6 beakers with 100 ml of water
  2. Fill beaker 1 with glucose enough for 1 m concentration using 100ml
  3. Fill beaker 2 with glucose enough for 0.8 m concentration using 100ml
  4. Fill beaker 3 with glucose enough for 0.6 m concentration using 100ml
  5. Fill beaker 4 with glucose enough for 0.4 m concentration using 100ml
  6. Fill beaker 5 with glucose enough for 0.2 m concentration using 100ml
  7. Take 3 pieces of kiwi and weigh them
  8. Take the same 3 pieces and put them in the 100% concentrated beaker
  9. Repeat steps 8 and 9 for beakers 2-6 (6 is the pure water beaker)
  10. Take out the pieces after 30 minutes (use the timer)
  11. Shake off the excess water and weigh them
  12. Record the percentage change in weight

Results

Concentration Before weight After weight % change in W
1 m 18.06 g 17.87 g -0.0019 %
0.8 m 16.73 g 16.46 g -0.0027 %
0.6 m 21.02 g 21.34 g 0.0032 %
0.4 m 25.13 g 25.26 g 0.0013 %
0.2 m 23.69 g 19.36 g 0.0007 %
0 m 19.12 g 19.36 g 0.0024 %

 

Conclusion

We have concluded that the isotonic point of kiwi to be 0.36 moles making it the juiciest fruit, potatoes coming in second with 0.42 moles, apples third with 0.61 moles, dragonfruit and mango both being 4rth at 0.78 moles, and melons coming in last with. 0.94 moles. The isotonic point of kiwi was found through the graph because the value the line cut the x-axis at is the isotonic point. It could also be observed through the graph that the relationship between x and y being negative. I have also observed that the pieces of kiwi fruit would expand and break apart in the 0 mole solution because it took in so much water, and the pieces in the 1 m solution shriveled. The changes weren’t dramatic at all because plants have cell walls making them pretty strong and sturdy.

Analysis

I had a couple errors during my experiment. We used tap water intead of distilled water, therefore it was not gauranteed that the water was 100% water. Also we used a electronic balance that only went until 3 decimal points therefore, all our weighed calculations are flawed. I feel like we havenn’t completely shaken off all the excess water, having miscalculations. Also we have controlled the experiment so the only forces in the water was osmosis by using sugar instead of salt. The beakers have been shaken by accident during the soaking period too. It is obvious that we had many errors beacuse in our graph it isn’t a perfect negative linear line but a negative zig zag, and also there is one dot that is way off course. Also we drew a line of best fit therefore our isotonic point is an estimate.

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